Calibration of Gas Chromatography (GC) : Pharmaceutical Guidelines

Calibration of Gas Chromatography (GC)

Learn how to calibrate the Gas Chromatography (Carrier Gas Flow, Column oven temperature and Detector calibration).

Calibration of Carrier Gas Flow Setting

Touch on “tool” at the right-hand corner of the screen on the instrument by using stick provided with the instrument.
Select option utilities.
Touch on column flow calc.
Enter column length, diameter, flow and column oven temperature; it will display required pressure to set by using carrier pressure knob.

Column oven temperature calibration

Ware goggle and gloves while handling glass wool.
Insert the calibrated digital thermometer from topside by piercing glass wool.
Set column oven temperature on GC to 50°C, 100°C, 200°C, and 250°C.
Allow to stabilize the set temperature for 5 min and record temperature on the digital thermometer at respective temperatures.
Frequency: Once in three months.
Related: Calibration of HPLC

Detector Calibration

Calibration of detector
Attach stainless steel packed column of length 2 M x diameter 1/8 inch with the stationary phase
10 % C-20 M (Ch-W/HP) 80/100
Prepare 1.0% v/v toluene solution in n-Hexane
Set the chromatographic conditions as follows
Injector temperature: 180°C
Detector temperature: 220°C
Column flow: 2.7 ml/min
Oven temperature: 110°C (isothermal)
Run Time: 5 minutes
Inject blank and then six injections of 1.0% toluene solution in system and record chromatogram for all injections
Calculate the RSD for the area of six 1.0 % toluene injections.
The RSD should not be more than 15.0%

Frequency: Once in six months.
SET TEMPERATURE ON GC
OBSERVED TEMPERATURE ON DIGITAL THERMOMETER
ACCEPTANCE CRITERIA
50°C
        48-52°C
100°C
        98-102°C
200°C
        198-202°C
250°C
        248-252°C
Also see: Principle of HPLC

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Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
.moc.enilediugamrahp@ofni :liamENeed Help: Ask Question


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2 comments: Post Yours! Read Comment Policy ▼

  1. please suggest how to calculate retention time of 6 inj. of toluene in GC calibration

    ReplyDelete
  2. sir pls tell us what is RSD FOR area in chromatogram

    ReplyDelete


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