Preparation and Standardization of 0.1 M Disodium Edetate (EDTA) : Pharmaguideline

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Preparation and Standardization of 0.1 M Disodium Edetate (EDTA)

Preparation and standardisation the 0.1 M Disodium Edetate (EDTA) solution using Calcium Carbonate and Hydroxy Nephthol Blue as indicator.

Disodium Edetate Solution Preparation

  • Take about 100 ml of water in a cleaned and dried 1000 ml volumetric flask.
  • Add about 37.2 gm of Disodium Edetate with continues stirring. 
  • Add more about 700 ml of water mix. 
  • Make up the volume 1000 ml with water. Mix the solution thoroughly. 
  • Keep the solution for at least one hour and then carry out the standardization.
Related: Determination of Shelf Life of Solutions in Laboratory

Disodium Edetate Solution Standardization

  • Weigh accurately about 400 mg of Calcium Carbonate.
  • Previously dried at about 110°C for about 2 hours.
  • Transfer it in a cleaned and dried 500 ml conical flask and add about 10 ml of water with gentle stirring to form the slurry. 
  • Dissolve the carbonate in 2 ml of dilute hydrochloric acid and shake well until it dissolves.
  • Add 100 ml water through the inner surface of the flask with the stirring of the solution.
  • Add 30 ml of 0.1 M Disodium Edetate (prepared) from the burette with constant stirring.
  • Add 15 ml of Sodium Hydroxide solution with constant stirring.
  • Add 300 mg of Hydroxy Naphthol Blue solution as an indicator
  • Add 0.1 M Disodium Edetate (prepared) with constant stirring and titrate it until the solution gives a blue color.
  • Calculate the molarity by the following formula:
                 W x 1000
M = ----------------------
               BR x 100.09

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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