Preparation and Standardization of 0.1 M Disodium Edetate (EDTA) : Pharmaceutical Guidelines

Preparation and Standardization of 0.1 M Disodium Edetate (EDTA)

Preparation and standardisation the 0.1 M Disodium Edetate (EDTA) solution using Calcium Carbonate and Hydroxy Nephthol Blue as indicator.

Disodium Edetate Solution Preparation:

•  Take about 100 ml of water in a cleaned and dried 1000 ml volumetric flask.
•  Add about 37.2 gm of Disodium Edetate with continues stirring.
•  Add more about 700 ml of water mix.
•  Make up the volume 1000 ml with water. Mix the solution thoroughly.
•  Keep the solution for at least one hour and then carry out the standardization.

Related: Determination of Shelf Life of Solutions in Laboratory

Disodium Edetate Solution Standardization:

•  Weigh accurately about 400 mg of Calcium Carbonate.
•  Previously dried at about 110°C for about 2 hour.
•  Transfer it in a cleaned and dried 500 ml conical flask and add about 10 ml of water with gentle stirring to form slurry.
•  Dissolve the carbonate in 2 ml of dilute hydrochloric acid and shake well until it dissolve.
•  Add 100 ml water through inner surface of the flask with the stirring of solution.
•  Add 30 ml of 0.1 M Disodium Edetate (prepared) from the burette with constant stirring.
•  Add 15 ml of Sodium Hydroxide solution with constant stirring.
•  Add 300 mg of Hydroxy Naphthol Blue solution as a indicator.
•  Add 0.1 M Disodium Edetate (prepared) with constant stirring and titrate it until the solution gives blue color.
•  Calculate the molarity by following formula:

                                                                         W x 1000    
 Molarity of 0.1 M Disodium Edetate =  ----------------------
                                                                        BR x 100.09

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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