Assay of Ascorbic acid : Pharmaceutical Guidelines

Assay of Ascorbic acid

Learn how to determine the assay of Ascorbic acid using Iodine solution and Starch as indicator.

Reagents Required

1.0M Sulfuric acid
0.05M Iodine

Procedure

Weigh accurately about 0.1 gm of the sample and dissolve in a mixture of 100 ml freshly boiled and cooled water and 25 ml of 1M sulfuric acid. Immediately titrate with 0.05M iodine, using the starch solution as indicator until a persistent blue-violet color is obtained. Each ml of 0.05M iodine is equivalent to 0.008806 gm of ascorbic acid.
Calculation
                                         BR X Actual molarity X 0.008806 X 100
% of Ascorbic acid = ----------------------------------------------------
                                                      0.05 X Wt. of Sample
BR= Burette Reading





Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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