Determination of Assay of Oxygen : Pharmaguideline

Online GMP Courses with Certificate


Determination of Assay of Oxygen

Learn how to determine the Assay of Oxygen in samples using mercury reservoir.


The apparatus shown in Fig comprises a gas burette of the type described under assay of nitrous which tube B is connected to a gas pipette comprising two bulbs of suitable size.


Charge the pipette with the reagents specified in the monograph. With the two-way tap open to tube B, draw the solution just to the level of the tap by moving the mercury reservoir. Open the two-way tap to tube A, completely fill the burette and tube A with mercury and close the two-way tap.

Connect a rubber tube to the exit valve of the cylinder of the gas under examination through a suitable pressure reducing device and pass a current of the gas through the tube for 1 minute. Whilst the gas is still flowing, connect the rubber tube to tube

A, immediately open the two-way tap to tube A, allow the specified quantity of the gas to enter the burette by lowering the mercury reservoir and close the two-way tap. Increase the pressure of the gas by raising the mercury reservoir, open the two-way tap to tube B and transfer all the gas to the pipette.

Close the tap and gently shake the pipette. After 15 minutes, when most of the gas has been absorbed by the liquid, draw the residual gas back into the burette and repeat the procedure beginning at the words "Increase the pressure of the gas .." until the volume of residual gas is constant. Measure the volume of the residual gas in the burette.

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
.moc.enilediugamrahp@ofni :liamENeed Help: Ask Question

No comments:

Post a Comment

Please don't spam. Comments having links would not be published.

Popular Categories

QA SOPs QC SOPs Micro SOPs HVAC Production SOPs Stores SOPs Checklists Maintenance SOPs HPLC Sterile GLP Validation Protocols Water System GDP Regulatory Maintenance Calibration Warning Letters Education B.Pharmacy
Online Courses

Follow Pharmaguideline



Editable Pharmaceutical Documents in MS-Word Format. Ready to use SOPs, Protocols, Master Plans, Manuals and more...



Recent Posts