Assay of Calcium Citrate : Pharmaceutical Guidelines

Assay of Calcium Citrate

Learn how to determine the assay of Calcium Citrate by Disodium Edetate and Hydroxy Naphthol Blue as indicator.

Reagent required

3N Hydrochloric acid
0.05M Disodium Edetate
Sodium hydroxide solution
Hydroxy naphthol blue


Dissolve about 350 mg of Calcium Citrate, previously dried at 150°C to constant weight and accurately weighed, in a mixture of 10 ml of water and 2 ml of 3N hydrochloric acid, and dilute with water to about 100 ml. While stirring, add about 30 ml of 0.05 M Edetate Disodium from a 50 ml burette. Add 15 ml of 1N sodium hydroxide and 300 mg of hydroxy naphthol blue as an indicator, and continue the titration to a blue endpoint. Each ml of 0.05 M Edetate Disodium is equivalent to 8.307 mg of Ca3(C6H5O7)2.

                      V x M x F                 100
% Assay = -------------------- x----------------
                      0.05 x W               (100-LOD)
V = Blank reading – Sample reading
M = Molarity of 0.05M Disodium Edetate
F = Factor
W = Weight of sample

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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  1. What is the effect of adding sodium hydroxide after 30ml EDTA


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