Preparation and Standardization of 0.1 M Hydrochloric acid (HCl) : Pharmaceutical Guidelines

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Preparation and Standardization of 0.1 M Hydrochloric acid (HCl)

Preparation and standardisation of 0.1 M Hydrochloric acid (HCl) using THAM (Tris (hydroxymethyl)-amino methane and Bromocresol Green indicator.

Hydrochloric acid Solution Preparation

  • Take about 100 ml of water in a cleaned and dried 1000 ml volumetric flask.
  • Add about 8.5 ml of Conc. Hydrochloric acid with continuous stirring. 
  • Add more about 700 ml of water, mix and allow to cool to room temperature. 
  • Make up the volume 1000 ml with water. Mix solution thoroughly. 
  • Keep the solution for at least one hour and then carry out the standardization.
Related: Determination of Shelf Life of Solutions in Laboratory

Hydrochloric acid Solution Standardization

  • Accurately weigh about 0.5 g of THAM (Tris (hydroxymethyl)-amino methane (tromethamine), previously dried at 105° for 3 hours and cooled in a desiccator, transfer to a conical flask.
  • Dissolve in 50 ml of distilled water.
  • Add 2 drops of Bromocresol Green indicator.
  • Titrate with 0.1M Hydrochloric acid to a pale yellow endpoint.
  • Each 121.14 mg of tromethamine is equivalent to 1 ml of 0.1M hydrochloric acid.
  • Calculate the molarity by the following formula:
           THAM in mg
M = ---------------------
        121.14 x HCl in ml





Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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