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SOP for Calibration of HPLC Column Oven


Standard operating procedure for the calibration of HPLC Column Oven using calibrated digital thermometer.

1.0  Objective 

       To provide a standard operating procedure for the calibration of HPLC Column Oven.

2.0  SCOPE

       This procedure is applicable to all HPLC Column Oven in QC Lab.   

3.0  Responsibility

3.1  Doing      : Technical Assistant/Executive
3.2  Checking : Executive /Manager   

4.0  Accountability

       Head of the Department

5.0  Procedure

       Frequency  : Once in a year.

5.1  Calibration Procedure for HPLC Column Oven

5.1.1  Keep the sensor probe of  reference calibrated digital thermometer in side the HPLC Column Oven keep it’s position in about centre of oven chamber.
5.1.2  Set the oven temp. at 25 oC .
5.1.3  Let the oven chamber to achieve set temp. Allow to be stabilize it for at least 5 min.
5.1.4  After 5 minutes, observe the temp. in ref. thermometer.
5.1.5  Record the value of temp. shown on instrument display and ref. thermometer in Annexure–I.
5.1.6  Keep minimum five minutes interval for first and second reading.
5.1.7  Then set the oven temp. 40°C, 50°C, 60°C and 70°C each one by one and follow the procedure as mentioned in step No. 5.1.3 to 5.1.6.  

6.0  Abbreviations

6.1  oC= Degree centigrade.
6.2  HPLC = High performance liquid Chromatography.

Related: Calibration of HPLC
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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