SOP for Calibration of HPLC Column Oven

1.0 Objective

To provide a standard operating procedure for the calibration of HPLC Column Oven.

2.0 Scope

This procedure is applicable to all HPLC Column Oven in QC Lab.

3.0 Responsibility

3.1 Doing: Technical Assistant/Executive
3.2 Checking: Executive /Manager

4.0 Accountability

Head of the Department

5.0 Procedure

Frequency: Once in a year.
5.1 Calibration Procedure for HPLC Column Oven
5.1.1 Keep the sensor probe of reference calibrated digital thermometer inside the HPLC Column Oven keeps its position in about center of the oven chamber.
5.1.2 Set the oven temp. at 25 °C.
5.1.3 Let the oven chamber to achieve set temp. Allow to stabilize it for at least 5 min.
5.1.4 After 5 minutes, observe the temp. in ref. thermometer.
5.1.5 Record the value of temp. shown on instrument display and ref. the thermometer in Annexure–I.
5.1.6 Keep minimum five minutes interval for first and second reading.
5.1.7 Then set the oven temp. 40°C, 50°C, 60°C and 70°C each one by one and follow the procedure as mentioned in step No. 5.1.3 to 5.1.6.

6.0 Abbreviations

6.1 °C= Degree centigrade.
6.2 HPLC = High performance liquid Chromatography.

Related: Calibration of HPLC

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